CARR® Centritech Separation Systems: CARR VP Pilot
Pneumatic Scale Angelus' CARR VP Pilot combines fast, gentle, small- to medium-scale cell harvesting and supernatant recovery for shear cells such as mammalian and insect cells and high G-force for liquid/solid separations of bacteria, yeast, proteins or submicron particles.
Viafuge Bowl For General Operation
Cells are concentrated at the bowl wall, while clarified supernatant is continuously discharged throughout the separation process. Once the bowl fills with cell concentrate to a capacity of 1.3L, a simple manifold system and concentrate pump is utilized to recover the cells without breaking containment. Subsequent cycles are performed for recovery of larger batches.
Improved Product Processing
Cellular damage can result in release of intracellular material such as DNA, which may increase downstream processing and, in some cases, impact product purity. Because the VP Pilot is gentle to cells, damage is minimized from the start of the process. For supernatant recovery applications, filtration requirements downstream may be reduced. For cell harvesting applications, intact cell recovery will be increased.
The VP Pilot’s unique design minimizes shear forces on cells. Incoming feed is matched to the bowl hub’s rotational velocity, allowing fast, efficient harvesting of even the most shear-sensitive cells without causing cell damage.
Flexible operating conditions offer optimization for specific applications.
- Feed rate 1 LPM
- G Force - UFD up to 20,000 x G
- Capacity - 1 liter solids space
- Temperature control - Jacketed and vacuum