Viafuge ® Pilot

Product Overview

Clean-in-Place / Sterilize-in-Place System

The Viafuge Pilot is designed for fast, gentle, small- to medium-scale cell harvesting and supernatant clarification. Capable of separating a wide variety of mammalian and insect cells with little to no damage, the Viafuge Pilot offers high purity results with undetectable cell concentrations in clarified supernatant.

Bowl capacity: 1.4 L
Flow rates up to 240 L/hr for batches up to 500 L
Footprint:

Centrifuge: 35.5 cm wide x 53.3 cm deep x 76.2 cm high ( 14” x 21” x 30”)
Cabinet: 40.6 cm wide x 40.6 cm deep x 76.2 cm high (16” x 16” x 30”)

Total weight ~ 136 kg (300 lbs)
Fully scalable to larger automated V12 models
Gentle cell harvesting and supernatant recovery
Improved product processing

Request Information

Low-Shear Performance

The Viafuge Pilot’s unique design minimizes shear forces on cells, boasting a typical cell viability of 98% or higher and up to 98% yield.

Scalability

Processes developed on the Viafuge Pilot can be seamlessly scaled to the Viafuge V12.

Features

Bowl capacity: 1.4L
Flow rates up to 240 L/hr for batches up to 500 L
Variable speeds up to 10,000 x g
SIP optional
cGMP construction
ASME-rated designs available
Low-shear design
Flexible optimization
Optional Hazardous Duty (Class 1, Div 1, Group C and D) and CE configurations are available
State-of-the-art manufacturing facility located in the United States

How It Works

Cells are concentrated at the bowl wall while clarified supernatant is continuously discharged throughout the separation process. Once the bowl fills with cell concentrate, a simple manifold system and concentrate pump is used to recover the cells without breaking containment. Subsequent cycles are performed for recovery of larger batch sizes.

Automation and General Specifications

Allen Bradley® or Siemens® PLC
Allen Bradley® or Siemens® HMI touchscreen 30.7 cm (12.1”)
Maximum case pressure (for cooling) 6.9 bar (100 psi)

Utility Requirements

Power configuration: Three phase voltage supply:

US: 230V/60 Hz, 20 A
Europe: 200V/50 Hz, 20 A
Japan: 200V/60 Hz/50 Hz, 20 A

Optional: Others as needed
Pneumatic: Clean, dry air supply 6 bar (90 psi) at 1 SLPM

Process Wetted Parts Construction

USP Class VI components

316L Stainless Steel
17-4 PH Stainless Steel
Nitronic 60 Stainless Steel
Platinum cured silicone hoses
EPDM elastomers
Ti-6Al-4V Titanium

Stainless Steel Centrifuges Brochure

Endorsements

See What People Are Saying About Our Equipment

“The UniFuge was easy to scale-up, and the Viafuge enables us to recover viable cells at large scale, beyond what single-use can typically accomplish."

Cellular Agriculture Entrepreneur

“We replaced our bottle centrifuges with a single continuous Viafuge, saving cleaning, autoclaving, and manual manipulations."

Animal Vaccine Manufacturing Operations Manager

"Gentle handling of our cells at scale resulted in higher VLP recovery.”

Director of Operations, Vaccine Manufacturing

“The high g-force of the Powerfuge [e. Coli lysate clarification] reduced our downstream clarification needs.”

Senior Process Development Scientist, pDNA

“Removing large particles with Powerfuge reduced the number of filtration steps.”

Manufacturing Engineer, Pigments

“The Powerfuge closed up our ethanol process and its automatic scraping improved our recovery.”

Associate Director of Vaccine Franchise

“The explosion-proof Powerfuge closed up our solvent process for a safe and environmentally friendly manufacturing method.”

Senior Process Engineer, Pigments